C# Email Regex - Blacklist characters such as " -

i have the following regex works validate email:

[a-za-z0-9!#$%&'*+/=?^_`{|}~-]+(?:\.[a-za-z0-9!#$%&'*+/=?^_`{|}~-]+)*@(?:[a-za-z0-9](?:[a-za-z0-9-]*[a-za-z0-9])?\.)+[a-za-z0-9](?:[a-za-z0-9-]*[a-za-z0-9]) 

but want add regex make fail if part of email contains characters such ".

if add

(?<!".*) 

to front of current regex expression, regex match fail if email address contains "